Cordyceps Sinesis
Hirsutella sinensis mycelium ( HSM) extract relieves pulmonary fibrosis though
suppress inflammation and function of Treg cells
Hirsutella sinensis mycelium ( HSM) extract relieves pulmonary fibrosis though
suppress inflammation and function of Treg cells
YUE Hui-Min ，LIU Fei ，LI Fan-Lin，MA Fei-Ya，SHEN Su-Nan，HOU Ya-Yi. Medical School ＆ State Key Laboratory
of Pharmaceutical Biotechnology，Nanjing University，Nanjing 210093，China
Abstract
and puncture ( CLP) . Methods: Both 5 day and 10 day timepoint pulmonary fibrosis were induced by CLP; mice were randomly divided
into three groups， including sham group ( sham，n = 5) ，model group ( CLP，n = 11) ， therapy group ( HSM，n = 11) . HSM extract
( 200 mg /kg， less than human dose of 0. 27 g /kg) was orally administered to HSM group 2 hour before surgery and repeated everyday，
while sham group and model group were given the same dose of normal saline. We used Q-PCＲ assay to analyze the expression of inflammatory
molecules ( IL-1β and TNF-α) and fibrogenic cytokines ( TGF-β，MMP9 and TIMP1) from lung tissues ，we used FACS assay
to analyze the amount of Th1 cells in PBMC and spleen sections， as well as Treg in spleen sections. Besides, lung sections were subjected
to HE stain and immunohistochemical staining with α-SMA and fibronectin. Ｒesults: The amount of model group's Th1 cells in
PBMC and spleen sections， as well as Treg in spleen sections were significantly lower than sham group's，whereas HSM succeeded in
raising them. By day 5，the expression of inflammatory molecules IL-1β and fibrogenic cytokines ( TGF-β，MMP9 and TIMP1) from
CLP group's lung sections were remarkably improved，by day 10， the expression had decreased but was higher than sham group's ; HSM
inhibited these cytokines' expression. The expression of TNF-α of CLP group and HSM group had no significant changes． The CLP
group's HE results showed obvious pulmonary inflammatory damage，by day 10， the situation had improved，whereas HSM reduced the
histopathologic alterations; contrast to sham group，model group's expression of α-SMA and fibronectin was remarkably improved，while
HSM group showed lower expression.
Conclusion: HSM extract can suppress inflammation，balances immune system and relieves pulmonary
About the author: Yue Huimin (1991-), female, master, mainly engaged in cellular and molecular immune research, E-mail: yuehuimin914 @ 126. com. Corresponding author and instructor: Hou Yayi (1960-), male, professor, doctoral tutor, mainly engaged in research on cellular and molecular immunity, E-mail: yayihou @ nju edu. cn.